Molecular Diversity among Sorghum (Sorghum bicolor (L.) Moench) Landraces in Uganda

Simple item page
dc.contributor.authorMbeyagala, Emmanuel K
dc.contributor.authorKiambi, Dan D
dc.contributor.authorOkori, Patrick
dc.contributor.authorEdema, Richard
dc.date.accessioned2024-01-22T13:25:33Z
dc.date.available2023-06-30T05:40:29Z
dc.date.available2024-01-22T13:25:33Z
dc.date.issued2012
dc.description.abstractThe variability in sorghum germplasm is an invaluable input for sustaining and improving sorghum productivity. A wide range of variability in phenotypic traits exists among landraces in Uganda. However, the diversity of the germplasm at the molecular level is not described and therefore not known which hinders its use in modern plant improvement programs. This study was therefore undertaken to classify 241 sorghum accessions collected from different agro-ecological regions based on genetic distances estimated using 21 Simple Sequence Repeat (SSR) markers. The SSR primers were highly polymorphic with average Polymorphic Information Content (PIC) of 0.65 ranging from 0.09-0.89. A total of 205 alleles (9.8 alleles per locus) as well as a number of rare alleles were observed across all the accessions and this provides an opportunity for generation of a comprehensive fin' gerprint database. Gene diversity ranged from 0.09-0.90 with an average of 0.68. The average heterozygosity detected was 0.18 ranging from 0.00-92%. Analysis of molecular variation showed that variation was higher within races and agro-ecologies than among races and agro-ecological zones, respectively and this indicated the significance of gene flow. Cluster analysis delineated the accessions into to two distinct clusters each with seven sub-clusters main' ly according to agro-ecological zone. Clusters IA and IB had the most distill' ct accessions and these could be utilized in. pre-breeding programmes aimed at overcoming yield barriers. The results confirm the ability of SSR markers to discern variability and also serve as guide for germplasm collection and conservation strategies.en_US
dc.identifier.issn1811-9700
dc.identifier.urihttps://repo.pacuniversity.ac.ke/handle/123456789/3694.2
dc.language.isoenen_US
dc.publisherInternational Journal of Botanyen_US
dc.subjectSorghum, SSR markers, PIC, landrace, diversityen_US
dc.titleMolecular Diversity among Sorghum (Sorghum bicolor (L.) Moench) Landraces in Ugandaen_US
dc.typeArticleen_US
Files
Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
Molecular Diversity among Sorghum (Sorghum bicolor (L.) Moench).pdf
Size:
986.66 KB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
JA-Department of Community Development

Version History

Now showing 1 - 1 of 1
VersionDateSummary
2*
 2024-01-22 13:24:54
n.v
* Selected version